ISO 20122:2024
(Main)Vegetable oils — Determination of mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH) with online-coupled high performance liquid chromatography-gas chromatography-flame ionization detection (HPLC-GC-FID) analysis — Method for low limit of quantification
Vegetable oils — Determination of mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH) with online-coupled high performance liquid chromatography-gas chromatography-flame ionization detection (HPLC-GC-FID) analysis — Method for low limit of quantification
This document specifies a procedure for the determination of saturated and aromatic hydrocarbons (from C10 to C50) in vegetable fats and oils using the online-coupled high performance liquid chromatography-gas chromatography-flame ionization detection (HPLC-GC-FID). This document does not apply to other matrices. The method is applicable for the analysis of mineral oil saturated hydrocarbons (MOSH) and/or mineral oil aromatic hydrocarbons (MOAH). According to the results of the interlaboratory studies, the method has been proven suitable for MOSH mass concentrations above 3 mg/kg and MOAH mass concentrations above 2 mg/kg. In case of suspected interferences, the fossil origin of the MOSH and MOAH fraction can be verified by examination by GC⨯GC-MS. An alternative method for the epoxidation of the MOAH fraction (performic acid epoxidation) is proposed in Annex C. This alternative method provides comparable results to the ethanolic epoxidation of the MOAH fraction described in 8.6. This alternative method for epoxidation has proven to be efficient for samples with a high amount of interferences in the MOAH fraction (e.g. tropical oils).
Huiles végétales — Dosage des hydrocarbures saturés d’huile minérale (MOSH) et des hydrocarbures aromatiques d’huile minérale (MOAH) par analyse par chromatographie en phase liquide haute performance et chromatographie en phase gazeuse couplées à un détecteur à ionisation de flamme (CLHP-CG-FID) en ligne — Méthode pour une faible limite de quantification
Le présent document spécifie un mode opératoire pour le dosage des hydrocarbures saturés et aromatiques (de C10 à C50) dans les matières grasses et huiles végétales en utilisant la chromatographie en phase liquide haute performance et la chromatographie en phase gazeuse couplées à un détecteur à ionisation de flamme (CLHP-CG-FID) en ligne. Le présent document ne s’applique pas à d’autres matrices. La méthode s’applique à l’analyse des hydrocarbures saturés d’huile minérale (MOSH) et/ou des hydrocarbures aromatiques d’huile minérale (MOAH). D’après les résultats des études interlaboratoires, il a été démontré que la méthode est adaptée pour des concentrations massiques de MOSH supérieures à 3 mg/kg et des concentrations massiques de MOAH supérieures à 2 mg/kg. En cas de suspicion d’interférences, l’origine fossile des fractions MOSH et MOAH peut être vérifiée par un examen par chromatographie en phase gazeuse à deux dimensions, couplée à la spectrométrie de masse (CG⨯CG-SM). Une méthode alternative d’époxydation de la fraction MOAH (époxydation par l’acide performique) est proposée dans l’Annexe C. Cette méthode donne des résultats comparables à l’époxydation éthanolique de la fraction MOAH décrite en 8.6. Cette méthode alternative d’époxydation s’est avérée efficace pour les échantillons présentant de fortes interférences dans la fraction MOAH (par exemple, les huiles tropicales).
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International
Standard
ISO 20122
First edition
Vegetable oils — Determination of
2024-04
mineral oil saturated hydrocarbons
(MOSH) and mineral oil aromatic
hydrocarbons (MOAH) with
online-coupled high performance
liquid chromatography-gas
chromatography-flame ionization
detection (HPLC-GC-FID) analysis
— Method for low limit of
quantification
Reference number
ISO 20122:2024(en) © ISO 2024
---------------------- Page: 1 ----------------------
ISO 20122:2024(en)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
© ISO 2024 – All rights reserved
ii
---------------------- Page: 2 ----------------------
ISO 20122:2024(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 3
6 Apparatus . 6
7 Sample . 7
7.1 Sampling .7
7.2 Preparation of the final sample for liquid and solid fats .7
8 Procedures . 8
8.1 General .8
8.2 Hexane/ethanol distribution for removal of interfering substances .8
8.3 Saponification . .8
8.4 Removal of biogenic n-alkanes with aluminium oxide for determination of the MOSH
fraction .9
8.5 Clean-up before epoxidation to separate po
...
International
Standard
ISO 20122
First edition
Vegetable oils — Determination of
mineral oil saturated hydrocarbons
(MOSH) and mineral oil aromatic
hydrocarbons (MOAH) with
online-coupled high performance
liquid chromatography-gas
chromatography-flame ionization
detection (HPLC-GC-FID) analysis
— Method for low limit of
quantification
PROOF/ÉPREUVE
Reference number
ISO 20122:2024(en) © ISO 2024
---------------------- Page: 1 ----------------------
ISO 20122:2024(en)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
PROOF/ÉPREUVE
© ISO 2024 – All rights reserved
ii
---------------------- Page: 2 ----------------------
ISO 20122:2024(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 3
6 Apparatus . 6
7 Sample . 7
7.1 Sampling .7
7.2 Preparation of the final sample for liquid and solid fats .7
8 Procedures . 8
8.1 General .8
8.2 Hexane/ethanol distribution for removal of interfering substances .8
8.3 Saponification . .8
8.4 Removal of biogenic n-alkanes with aluminium oxide for determination of the MOSH
fraction .9
8.5 Clean-up before epoxidat
...
© ISO 2023 – All rights reserved
ISO/DIS PRF 20122:2023(E)
Date: 2024-01-30
ISO/TC 34/SC 11
Secretariat: BSI
Date: 2024-02-20
Vegetable oils — Determination of mineral oil saturated
hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons
(MOAH) with online-coupled high performance liquid
chromatography-gas chromatography-flame ionization detection
(HPLC-GC-FID) analysis — Method for low limit of quantification
PROOF
---------------------- Page: 1 ----------------------
ISO/PRF 20122:2024(en)
© ISO 2024
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication
may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying,
or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO
at the address below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: + 41 22 749 01 11
E-mail: copyright@iso.org
Website: www.iso.org
Published in Switzerland
© ISO 2024 – All rights reserved
ii
---------------------- Page: 2 ----------------------
ISO/PRF 20122:2024(en)
Contents
Foreword . v
Introduction . vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 3
6 Apparatus . 6
7 Sample . 7
7.1 Sampling . 7
7.2 Preparation of the final sample for liquid and solid fats . 7
8 Procedures . 8
8.1 General . 8
8.2 Hexane/ethanol distribution for removal of interfering substances . 8
8.3 Saponification . 8
8.4 Removal of biogenic n-alkanes with aluminium oxide for determination of the MOSH fraction . 9
8.5 Clean-up before epoxidation to separate polar substances . 9
8.6 Ethanolic epoxidation of the MOAH fraction to oxidize unsaturated non-aromatic compounds . 9
8.7 HPLC-GC separation . 10
8.7.1 HPLC conditions . 10
8.7.2 GC configuration .
...
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